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Tissues were then incubated in 10, 20, and 30% sucrose in 1 PBS, embedded in OCT compound (Tissue-Tek, Sakura Finetek, 4583), snap frozen on dry ice, and then stored frozen at 80C
Free radicals generate, and Glutathione kicks into high gear to render those free radicals harmless
doi: 10.1053/j.gastro.2018.07.014 280
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